Journal: Cell metabolism

Article Title: Single-cell dissection of the obesity-exercise axis in adipose-muscle tissues implies a critical role for mesenchymal stem cells

doi: 10.1016/j.cmet.2022.09.004

Figure Lengend Snippet:

Article Snippet: Skeletal muscle tissue dissociation kit , Miltenyi Biotec , 130-098-305.

Techniques: Recombinant, RNAscope, Multiplex Assay, Sequencing, Software

Journal: Saudi Journal of Biological Sciences

Article Title: Effect of pioglitazone on skeletal muscle lipid deposition in the insulin resistance rat model induced by high fructose diet under AMPK signaling pathway

doi: 10.1016/j.sjbs.2020.03.014

Figure Lengend Snippet: Rat skeletal muscle fat drop oil red O staining (A. ND group; B. HFD group; C. Pioglitazone intervention group, ×400).

Article Snippet: Total RNA extracted from rat skeletal muscle tissues was used for cDNA reverse transcription according to PrimeScript™ RT reagent Kit with gDNA Eraser from TAKARA company.

Techniques: Staining

Journal: Saudi Journal of Biological Sciences

Article Title: Effect of pioglitazone on skeletal muscle lipid deposition in the insulin resistance rat model induced by high fructose diet under AMPK signaling pathway

doi: 10.1016/j.sjbs.2020.03.014

Figure Lengend Snippet: mRNA expression levels of target genes in skeletal muscle of rats (* indicates significant difference in expression compared with the HFD group, P < 0.05).

Article Snippet: Total RNA extracted from rat skeletal muscle tissues was used for cDNA reverse transcription according to PrimeScript™ RT reagent Kit with gDNA Eraser from TAKARA company.

Techniques: Expressing

Journal: Saudi Journal of Biological Sciences

Article Title: Effect of pioglitazone on skeletal muscle lipid deposition in the insulin resistance rat model induced by high fructose diet under AMPK signaling pathway

doi: 10.1016/j.sjbs.2020.03.014

Figure Lengend Snippet: mRNA and protein expression levels of AMPK and ACC genes in skeletal muscle of rats (A. Relative expression levels of mRNA and protein of APMK gene in skeletal muscle of rats under the intervention of ND, HFD, and pioglitazone; B. Relative expression levels of mRNA and protein of ACC gene in skeletal muscle of different groups of rats; C. Relative expression levels of protein of P-AMPK and P-ACC gene in skeletal muscle of different groups of rats; D. Western blotting map of P-AMPK, AMPK, P-ACC, ACC, and β-actin; * indicated that the expression was significantly different from the HFD group, P < 0.05 ).

Article Snippet: Total RNA extracted from rat skeletal muscle tissues was used for cDNA reverse transcription according to PrimeScript™ RT reagent Kit with gDNA Eraser from TAKARA company.

Techniques: Expressing, Western Blot

Journal: Saudi Journal of Biological Sciences

Article Title: Effect of pioglitazone on skeletal muscle lipid deposition in the insulin resistance rat model induced by high fructose diet under AMPK signaling pathway

doi: 10.1016/j.sjbs.2020.03.014

Figure Lengend Snippet: mRNA and protein expression levels of GLUT7, PGC-1α, and CPT1 genes in rat skeletal muscle (A. Relative expression levels of mRNA and protein of GLUT7 gene in rat skeletal muscle under the intervention of ND, HFD and pioglitazone; B. Relative expression levels of mRNA and protein of PGC-1α gene in skeletal muscle of rats in different groups; C. Relative expression levels of mRNA and protein of CPT1 gene in skeletal muscle of rats in different groups; D. Western blotting figure of GLUT7, PGC-1α, CPT1 and β-actin; * indicated that the expression was significantly different from that of the HFD group, P < 0.05 ).

Article Snippet: Total RNA extracted from rat skeletal muscle tissues was used for cDNA reverse transcription according to PrimeScript™ RT reagent Kit with gDNA Eraser from TAKARA company.

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: Statins exacerbate glucose intolerance and hyperglycemia in a high sucrose fed rodent model

doi: 10.1038/s41598-019-45369-8

Figure Lengend Snippet: Effect of statins on the lipid and gene expression profiles in skeletal muscle of HSD rats. ( A ) Simvastatin or Atorvastatin treatment enhanced free fatty acid levels in skeletal muscle tissue of HSD animals. HSD animals were treated orally with Atorvastatin (20 mg/kg/day) and Simvastatin (30 mg/kg/day) for 30 days. Animals were euthanized, skeletal muscle tissues were isolated and free fatty acid levels were measured. (B – D) Simvastatin or Atorvastatin treatment enhanced FAS, SREBP2 and ACC1 gene expression in muscle tissue of statins treated HSD animals : mRNA expression of rattus FAS, SREBP2 and ACC1 in statin muscle tissue of HSD animals. * p < 0.05, ** p < 0.01, *** p < 0.001 versus corresponding muscles of control HSD animals. (E) Simvastatin or Atorvastatin treatment inhibited insulin signalling cascade: Skeletal Muscles tissues were collected after statin treatment of HSD animals and probed by western blots for phosphorylation status of IRS-1 (ser 307 and ser 612 , tyr 608 ) and pAKT. Total IRS-1, total AKT and β-actin served as loading control. The cropped blots were run under the same experimental conditions. The full-length blots are included in Supplemental Fig. (Fig. . ( F–I ) Densitometric quantification results of western blots from ( E ). Values are shown as mean ± SD after normalizing with the corresponding protein content and expressed relative to basal (total AKT and β-tubulin) for pAKT and relative to basal (total IRS-1) for p-ser 307 , p-ser 612 and p-tyr 608 of muscle tissues of control HSD animals which was set to 1 versus muscle tissues of statin treated HSD animals.

Article Snippet: 100 mg rat skeletal muscle tissue was homogenized using RNA Isoplus reagent (Takara, Cat.no: 9108) and chloroform was added to the homogenate.

Techniques: Gene Expression, Isolation, Expressing, Muscles, Control, Western Blot, Phospho-proteomics